kpni (New England Biolabs)
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97
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New England Biolabs
kpni
Kpni, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1703 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/kpni/KpnI-HF/pmc13170675-221-60-62
Average 97 stars, based on 1703 article reviews
Kpni, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1703 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/kpni/KpnI-HF/pmc13170675-221-60-62
Average 97 stars, based on 1703 article reviews
kpni - by Bioz Stars,
2026-10
97/100 stars
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Blocking Assay:Article Title: An inducible CRISPRi system for phenotypic analysis of essential genes in Pseudomonas aeruginosa Article Snippet: .. The gene block and pUC18T-miniTn7T-pBAD-dcas9-Gm plasmid were restriction-digested with HindIII and Plasmid Preparation:Article Title: An inducible CRISPRi system for phenotypic analysis of essential genes in Pseudomonas aeruginosa Article Snippet: .. The gene block and pUC18T-miniTn7T-pBAD-dcas9-Gm plasmid were restriction-digested with HindIII and Article Title: Intrathecal delivery of AAVrh10-mHexa combined with anti-inflammatory treatment reduces neuropathological markers and extends the lifespan of mice with early-onset Tay-Sachs disease Article Snippet: Vector verification was performed through restriction enzyme mapping. .. EcoRI (Biolab NEB Cat# 0101 S), Article Title: Systematic multi-reference vertebrate ACE2 sequence similarity analysis predicts species susceptibility to SARS-related sarbecoviruses Article Snippet: Codon-optimized ACE2 ORF sequences were synthesized as gene fragments (mouse, pig, narwhal, reindeer, Coquerel’s sifaka, sinicus bat allele 1443, affinis bat), which contain 5’ KpnI and 3’ MluI restriction sites for downstream cloning into above described the pCMV6-humanACE2-MYC-FLAG backbone, or directly synthesized as complete expression plasmids (hedgehog, ruff, Himalayan frog, sinicus bat allele 3358) by Twist Bioscience. .. ACE2 gene fragments were cloned into Article Title: Compositions and methods for targeted delivery of CRISPR-Cas effector polypeptides and transgenes Article Snippet: In-Fusion (Takara Bio) cloning was used to clone Gag-Cas9 and Env into the pCAGGS expression vector. pCF221 (Addgene plasmid #121669) was modified to express mNeonGreen (Allele Biotechnology) or the α-CD19-4-1BBζ-P2A-mCherry (CAR-P2A-mCherry) construct (Hill et al., 2018; Muller et al., 2020) in place of mCherry and was used as the sgRNA-expressing lentiviral transfer plasmid. .. For generation of hybrid Cas9-VLPs, the guide RNA expression cassette was removed from the CAR-P2A-mCherry lentiviral plasmid via digestion with EcoRI and Article Title: Intrathecal delivery of AAVrh10-mHexa combined with anti-inflammatory treatment reduces neuropathological markers and extends the lifespan of mice with early-onset Tay-Sachs disease. Article Snippet: Vector verification was performed through restriction enzyme mapping. .. EcoRI (Biolab NEB Cat# 0101 S), Clone Assay:Article Title: An inducible CRISPRi system for phenotypic analysis of essential genes in Pseudomonas aeruginosa Article Snippet: .. The gene block and pUC18T-miniTn7T-pBAD-dcas9-Gm plasmid were restriction-digested with HindIII and Article Title: Systematic multi-reference vertebrate ACE2 sequence similarity analysis predicts species susceptibility to SARS-related sarbecoviruses Article Snippet: Codon-optimized ACE2 ORF sequences were synthesized as gene fragments (mouse, pig, narwhal, reindeer, Coquerel’s sifaka, sinicus bat allele 1443, affinis bat), which contain 5’ KpnI and 3’ MluI restriction sites for downstream cloning into above described the pCMV6-humanACE2-MYC-FLAG backbone, or directly synthesized as complete expression plasmids (hedgehog, ruff, Himalayan frog, sinicus bat allele 3358) by Twist Bioscience. .. ACE2 gene fragments were cloned into Amplification:Article Title: A Wnt-induced conformational phospho-switch in DVL3 controls association with Frizzled receptors and Wnt/β-catenin signaling Article Snippet: .. hDVL3 segments of interest (IDR2, amino acids 335 to 396; DEP, amino acids 398 to 496; PDZ, amino acids 243 to 351; and PDZ-IDR2-DEP, amino acids 243 to 496) were amplified by polymerase chain reaction (PCR) and inserted into a pETM11 or pET-Z (IDR2) expression vectors via restriction digestion between the 5′-NcoI and Polymerase Chain Reaction:Article Title: A Wnt-induced conformational phospho-switch in DVL3 controls association with Frizzled receptors and Wnt/β-catenin signaling Article Snippet: .. hDVL3 segments of interest (IDR2, amino acids 335 to 396; DEP, amino acids 398 to 496; PDZ, amino acids 243 to 351; and PDZ-IDR2-DEP, amino acids 243 to 496) were amplified by polymerase chain reaction (PCR) and inserted into a pETM11 or pET-Z (IDR2) expression vectors via restriction digestion between the 5′-NcoI and Expressing:Article Title: A Wnt-induced conformational phospho-switch in DVL3 controls association with Frizzled receptors and Wnt/β-catenin signaling Article Snippet: .. hDVL3 segments of interest (IDR2, amino acids 335 to 396; DEP, amino acids 398 to 496; PDZ, amino acids 243 to 351; and PDZ-IDR2-DEP, amino acids 243 to 496) were amplified by polymerase chain reaction (PCR) and inserted into a pETM11 or pET-Z (IDR2) expression vectors via restriction digestion between the 5′-NcoI and Article Title: Intrathecal delivery of AAVrh10-mHexa combined with anti-inflammatory treatment reduces neuropathological markers and extends the lifespan of mice with early-onset Tay-Sachs disease Article Snippet: Vector verification was performed through restriction enzyme mapping. .. EcoRI (Biolab NEB Cat# 0101 S), Article Title: Systematic multi-reference vertebrate ACE2 sequence similarity analysis predicts species susceptibility to SARS-related sarbecoviruses Article Snippet: Codon-optimized ACE2 ORF sequences were synthesized as gene fragments (mouse, pig, narwhal, reindeer, Coquerel’s sifaka, sinicus bat allele 1443, affinis bat), which contain 5’ KpnI and 3’ MluI restriction sites for downstream cloning into above described the pCMV6-humanACE2-MYC-FLAG backbone, or directly synthesized as complete expression plasmids (hedgehog, ruff, Himalayan frog, sinicus bat allele 3358) by Twist Bioscience. .. ACE2 gene fragments were cloned into Article Title: Intrathecal delivery of AAVrh10-mHexa combined with anti-inflammatory treatment reduces neuropathological markers and extends the lifespan of mice with early-onset Tay-Sachs disease. Article Snippet: Vector verification was performed through restriction enzyme mapping. .. EcoRI (Biolab NEB Cat# 0101 S), Cloning:Article Title: Systematic multi-reference vertebrate ACE2 sequence similarity analysis predicts species susceptibility to SARS-related sarbecoviruses Article Snippet: Codon-optimized ACE2 ORF sequences were synthesized as gene fragments (mouse, pig, narwhal, reindeer, Coquerel’s sifaka, sinicus bat allele 1443, affinis bat), which contain 5’ KpnI and 3’ MluI restriction sites for downstream cloning into above described the pCMV6-humanACE2-MYC-FLAG backbone, or directly synthesized as complete expression plasmids (hedgehog, ruff, Himalayan frog, sinicus bat allele 3358) by Twist Bioscience. .. ACE2 gene fragments were cloned into Synthesized:Article Title: Systematic multi-reference vertebrate ACE2 sequence similarity analysis predicts species susceptibility to SARS-related sarbecoviruses Article Snippet: Codon-optimized ACE2 ORF sequences were synthesized as gene fragments (mouse, pig, narwhal, reindeer, Coquerel’s sifaka, sinicus bat allele 1443, affinis bat), which contain 5’ KpnI and 3’ MluI restriction sites for downstream cloning into above described the pCMV6-humanACE2-MYC-FLAG backbone, or directly synthesized as complete expression plasmids (hedgehog, ruff, Himalayan frog, sinicus bat allele 3358) by Twist Bioscience. .. ACE2 gene fragments were cloned into RNA Expression:Article Title: Compositions and methods for targeted delivery of CRISPR-Cas effector polypeptides and transgenes Article Snippet: In-Fusion (Takara Bio) cloning was used to clone Gag-Cas9 and Env into the pCAGGS expression vector. pCF221 (Addgene plasmid #121669) was modified to express mNeonGreen (Allele Biotechnology) or the α-CD19-4-1BBζ-P2A-mCherry (CAR-P2A-mCherry) construct (Hill et al., 2018; Muller et al., 2020) in place of mCherry and was used as the sgRNA-expressing lentiviral transfer plasmid. .. For generation of hybrid Cas9-VLPs, the guide RNA expression cassette was removed from the CAR-P2A-mCherry lentiviral plasmid via digestion with EcoRI and |